TY  - JOUR
TI  - From conjugation to T4S systems in Gram-negative bacteria: a mechanistic biology perspective
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N2  - Conjugation is the process by which bacteria exchange genetic materials in a unidirectional manner from a donor cell to a recipient cell. The discovery of conjugation signalled the dawn of genetics and molecular biology. In Gram-negative bacteria, the process of conjugation is mediated by a large membrane-embedded machinery termed "conjugative type IV secretion (T4S) system", a large injection nanomachine, which together with a DNA-processing machinery termed "the relaxosome" and a large extracellular tube termed "pilus" orchestrates directional DNA transfer. Here, the focus is on past and latest research in the field of conjugation and T4S systems in Gram-negative bacteria, with an emphasis on the various questions and debates that permeate the field from a mechanistic perspective.
AV  - public
VL  - 2018
KW  - DNA and Protein Secretion
KW  -  bacterial conjugation
KW  -  pilus biogenesis
KW  -  relaxosome
KW  -  type IV secretion system
Y1  - 2019/01/02/
ID  - discovery10065849
JF  - EMBO Reports
SN  - 1469-3178
UR  - https://doi.org/10.15252/embr.201847012
A1  - Waksman, G
ER  -