%0 Journal Article
%@ 1940-6029
%A Bowers, K
%D 2019
%F discovery:10079177
%J Methods in Molecular Biology
%K ESCRT, Endosomes, Protein depletion, RNAi, siRNA
%P 305-318
%T RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells
%U https://discovery-pp.ucl.ac.uk/id/eprint/10079177/
%V 1998
%X Specific depletion of proteins from cultured cells using RNA interference (RNAi) has  been a useful technique in assessing protein function for many years. RNAi allows  the degradation of specific, targeted mRNA, allowing the effects of protein depletion  on cellular processes to be examined. Here, I present a protocol for the depletion of  proteins from cultured HeLa cells, and list specific reagents and considerations for  targeting the endosomal sorting complexes required for transport (ESCRT).
%Z This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.