%0 Journal Article %@ 1940-6029 %A Bowers, K %D 2019 %F discovery:10079177 %J Methods in Molecular Biology %K ESCRT, Endosomes, Protein depletion, RNAi, siRNA %P 305-318 %T RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells %U https://discovery-pp.ucl.ac.uk/id/eprint/10079177/ %V 1998 %X Specific depletion of proteins from cultured cells using RNA interference (RNAi) has been a useful technique in assessing protein function for many years. RNAi allows the degradation of specific, targeted mRNA, allowing the effects of protein depletion on cellular processes to be examined. Here, I present a protocol for the depletion of proteins from cultured HeLa cells, and list specific reagents and considerations for targeting the endosomal sorting complexes required for transport (ESCRT). %Z This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.