eprintid: 10084811 rev_number: 22 eprint_status: archive userid: 608 dir: disk0/10/08/48/11 datestamp: 2019-11-01 17:03:40 lastmod: 2021-09-28 22:26:32 status_changed: 2019-11-01 17:03:40 type: article metadata_visibility: show creators_name: Wei, J creators_name: Cheng, L creators_name: Li, J creators_name: Liu, Y creators_name: Yin, S creators_name: Xu, B creators_name: Wang, D creators_name: Lu, H creators_name: Liu, C title: A microfluidic platform culturing two cell lines paralleled under in-vivo like fluidic microenvironment for testing the tumor targeting of nanoparticles ispublished: pub divisions: UCL divisions: B04 divisions: C05 divisions: F45 keywords: Microfluidic, Paralleled introducing, Microenvironment, Targeting, Nanoparticles note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Nanoparticles are attractive in medicine because their surfaces can be chemically modified for targeting specific disease cells, especially for cancer. Providing an in-vivo like platform is crucial to evaluate the biological behaviours of nanoparticles. This paper presents a microfluidic device that could culture two cell lines in parallel in in-vivo like fluidic microenvironments and be used for testing the tumor targeting of folic acid - cholesterol - chitosan (FACC) nanoparticles. The uniformity and uniformity of flow fields inside the cell culture units are investigated using the finite element method and particle tracking technology. HeLa and A549 cells are cultured in the microfluidic chip under continuous media supplementation, mimicking the fluid microenvironment in vivo. Cell introducing processes are presented by the flow behaviours of inks with different colours. The two cell lines are identified by detecting folate receptors on the cellular membranes. The growth curves of the two cell lines are measured. The two cell lines cultured paralleled inside the microfluidic device are treated with FITC-FACC to investigate the targeting of FACC. The tumor targeting of FACC are also detected by in vivo imaging of HeLa cells growth in nude mice models. The results indicate that the microfluidic device could provide a dynamic, uniform and stable fluidic microenvironment to test the tumor targeting of FACC nanoparticles. date: 2020-02-01 date_type: published official_url: https://doi.org/10.1016/j.talanta.2019.120355 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1702177 doi: 10.1016/j.talanta.2019.120355 lyricists_name: Liu, Yuanchang lyricists_id: YLIUA09 actors_name: Allington-Smith, Dominic actors_id: DAALL44 actors_role: owner full_text_status: public publication: Talanta volume: 208 article_number: 120355 citation: Wei, J; Cheng, L; Li, J; Liu, Y; Yin, S; Xu, B; Wang, D; ... Liu, C; + view all <#> Wei, J; Cheng, L; Li, J; Liu, Y; Yin, S; Xu, B; Wang, D; Lu, H; Liu, C; - view fewer <#> (2020) A microfluidic platform culturing two cell lines paralleled under in-vivo like fluidic microenvironment for testing the tumor targeting of nanoparticles. Talanta , 208 , Article 120355. 10.1016/j.talanta.2019.120355 <https://doi.org/10.1016/j.talanta.2019.120355>. Green open access document_url: https://discovery-pp.ucl.ac.uk/id/eprint/10084811/3/Liu_A%20microfluidic%20platform%20culturing%20two%20cell%20lines%20paralleled%20under%20in-vivo%20like%20fluidic%20microenvironment%20for%20testing%20the%20tumor%20targeting%20of%20nanoparticles.pdf