%D 2019 %X Chemical shifts are often the only nuclear magnetic resonance parameter that can be obtained for challenging macromolecular systems. Here we present a framework to derive the conformational sampling of isoleucine side chains from {13}^C chemical shifts and demonstrate that side-chain conformations in a low-populated folding intermediate can be determined. %T Determining isoleucine side-chain rotamer-sampling in proteins from {13}^C chemical shift %A L Siemons %A B Uluca-Yazgi %A RB Pritchard %A S McCarthy %A H Heise %A DF Hansen %C England %O © The Royal Society of Chemistry 2019. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/). %J Chemical Communications %L discovery10085106