TY  - JOUR
ID  - discovery10093058
JF  - Nature Cell Biology
Y1  - 2019/07//
A1  - Diring, J
A1  - Mouilleron, S
A1  - McDonald, NQ
A1  - Treisman, R
UR  - https://doi.org/10.1038/s41556-019-0337-y
SN  - 1476-4679
PB  - NATURE PUBLISHING GROUP
N2  - RPEL proteins, which contain the G-actin-binding RPEL motif, coordinate cytoskeletal processes with actin dynamics. We show that the ArhGAP12- and ArhGAP32-family GTPase-activating proteins (GAPs) are RPEL proteins. We determine the structure of the ArhGAP12/G-actin complex, and show that G-actin contacts the RPEL motif and GAP domain sequences. G-actin inhibits ArhGAP12 GAP activity, and this requires the G-actin contacts identified in the structure. In B16 melanoma cells, ArhGAP12 suppresses basal Rac and Cdc42 activity, F-actin assembly, invadopodia formation and experimental metastasis. In this setting, ArhGAP12 mutants defective for G-actin binding exhibit more effective downregulation of Rac GTP loading following HGF stimulation and enhanced inhibition of Rac-dependent processes, including invadopodia formation. Potentiation or disruption of the G-actin/ArhGAP12 interaction, by treatment with the actin-binding drugs latrunculin B or cytochalasin D, has corresponding effects on Rac GTP loading. The interaction of G-actin with RPEL-family rhoGAPs thus provides a negative feedback loop that couples Rac activity to actin dynamics.
VL  - 21
AV  - public
TI  - RPEL-family rhoGAPs link Rac/Cdc42 GTP loading to G-actin availability
N1  - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions.
EP  - 855
SP  - 845
ER  -