eprintid: 10096371
rev_number: 12
eprint_status: archive
userid: 608
dir: disk0/10/09/63/71
datestamp: 2020-04-27 15:49:40
lastmod: 2020-04-27 15:49:40
status_changed: 2020-04-27 15:49:40
type: article
metadata_visibility: show
creators_name: Hazael, R
creators_name: Matsuda, S
creators_name: Mori, Y
creators_name: Fitzmaurice, BC
creators_name: Appleby-Thomas, G
creators_name: Painter, JD
creators_name: Meersman, F
creators_name: Richaud, M
creators_name: Galas, S
creators_name: Saini, NL
creators_name: Ono, F
creators_name: McMillan, PF
title: Pressure tolerance of Artemia cysts compressed in water medium
ispublished: pub
divisions: UCL
divisions: A01
divisions: B04
divisions: C06
divisions: F56
keywords: Artemia salina, cysts, high pressure tolerance, hatchability, osmotic pressure, ice VI phase
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: The high pressure tolerance of cysts of Artemia salina was investigated up to several GPa in water. No survival was observed after exposure to 1.0 GPa for 15 min. After exposure to 2.0 GPa for the same time duration, the hatching rate had recovered to 33%, but decreased to 8% following compression at 7.5 GPa. This contrasts with results using Fluorinert™ as the pressure-transmitting medium where 80–88% recovery was observed. The lower survival rate in water is accompanied by swelling of the eggs, indicating that liquid H2O close to the ice-VI crystallization pressure penetrated inside the eggs. This pressure exceeds the stability limit for proteins and other key biomolecules components within the embryos that could not be resuscitated. Rehydration takes several minutes and so was not completed for all samples compressed to higher pressures, prior to ice-VI formation, resulting in renewed survival. However H2O penetration inside the shell resulted in increased mortality.
date: 2019-02-04
date_type: published
official_url: https://doi.org/10.1080/08957959.2019.1575378
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1629820
doi: 10.1080/08957959.2019.1575378
lyricists_name: McMillan, Paul
lyricists_id: PFMCM40
actors_name: Stacey, Thomas
actors_id: TSSTA20
actors_role: owner
full_text_status: public
publication: High Pressure Research
volume: 39
number: 2
pagerange: 293-300
citation:        Hazael, R;    Matsuda, S;    Mori, Y;    Fitzmaurice, BC;    Appleby-Thomas, G;    Painter, JD;    Meersman, F;                     ... McMillan, PF; + view all <#>        Hazael, R;  Matsuda, S;  Mori, Y;  Fitzmaurice, BC;  Appleby-Thomas, G;  Painter, JD;  Meersman, F;  Richaud, M;  Galas, S;  Saini, NL;  Ono, F;  McMillan, PF;   - view fewer <#>    (2019)    Pressure tolerance of Artemia cysts compressed in water medium.                   High Pressure Research , 39  (2)   pp. 293-300.    10.1080/08957959.2019.1575378 <https://doi.org/10.1080/08957959.2019.1575378>.       Green open access   
 
document_url: https://discovery-pp.ucl.ac.uk/id/eprint/10096371/1/McMillan_1.%20F%20Ono-HPR%20manuscript-revised%202019-1-18.pdf