eprintid: 10114820 rev_number: 39 eprint_status: archive userid: 608 dir: disk0/10/11/48/20 datestamp: 2020-11-13 18:24:52 lastmod: 2022-08-17 09:45:42 status_changed: 2020-11-13 18:24:52 type: article metadata_visibility: show creators_name: Ng, KW creators_name: Faulkner, N creators_name: Cornish, GH creators_name: Rosa, A creators_name: Harvey, R creators_name: Hussain, S creators_name: Ulferts, R creators_name: Earl, C creators_name: Wrobel, AG creators_name: Benton, DJ creators_name: Roustan, C creators_name: Bolland, W creators_name: Thompson, R creators_name: Agua-Doce, A creators_name: Hobson, P creators_name: Heaney, J creators_name: Rickman, H creators_name: Paraskevopoulou, S creators_name: Houlihan, CF creators_name: Thomson, K creators_name: Sanchez, E creators_name: Shin, GY creators_name: Spyer, MJ creators_name: Joshi, D creators_name: O'Reilly, N creators_name: Walker, PA creators_name: Kjaer, S creators_name: Riddell, A creators_name: Moore, C creators_name: Jebson, BR creators_name: Wilkinson, M creators_name: Marshall, LR creators_name: Rosser, EC creators_name: Radziszewska, A creators_name: Peckham, H creators_name: Ciurtin, C creators_name: Wedderburn, LR creators_name: Beale, R creators_name: Swanton, C creators_name: Gandhi, S creators_name: Stockinger, B creators_name: McCauley, J creators_name: Gamblin, SJ creators_name: McCoy, LE creators_name: Cherepanov, P creators_name: Nastouli, E creators_name: Kassiotis, G title: Preexisting and de novo humoral immunity to SARS-CoV-2 in humans ispublished: inpress subjects: GOSH subjects: UCH divisions: UCL divisions: B02 divisions: C10 divisions: D19 divisions: G99 divisions: D15 divisions: D17 divisions: G90 divisions: G93 divisions: D13 divisions: G24 note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detect preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable by a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the IgG class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies, targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. Notably, SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection. date: 2020-11-06 date_type: published official_url: https://dx.doi.org/10.1126/science.abe1107 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1828141 doi: 10.1126/science.abe1107 pii: science.abe1107 lyricists_name: Beale, Rupert lyricists_name: Ciurtin, Coziana lyricists_name: Houlihan, Catherine lyricists_name: Jebson, Bethany-Rose lyricists_name: McCoy, Laura lyricists_name: Nastouli, Eleni lyricists_name: Peckham, Hannah lyricists_name: Radziszewska, Anna lyricists_name: Rosser, Elizabeth lyricists_name: Spyer, Moira lyricists_name: Swanton, Robert lyricists_name: Wedderburn, Lucy lyricists_name: Wilkinson, Meredyth lyricists_id: RBEAL65 lyricists_id: CCIUR23 lyricists_id: CFHOU59 lyricists_id: BJEBS56 lyricists_id: LEFMC71 lyricists_id: ENAST37 lyricists_id: HLPEC87 lyricists_id: ARADZ72 lyricists_id: EROSS73 lyricists_id: MJSPY20 lyricists_id: CSWAN79 lyricists_id: WLUCY30 lyricists_id: MGLWI52 actors_name: Wedderburn, Lucy actors_id: WLUCY30 actors_role: owner full_text_status: public publication: Science article_number: eabe1107 event_location: United States citation: Ng, KW; Faulkner, N; Cornish, GH; Rosa, A; Harvey, R; Hussain, S; Ulferts, R; ... Kassiotis, G; + view all <#> Ng, KW; Faulkner, N; Cornish, GH; Rosa, A; Harvey, R; Hussain, S; Ulferts, R; Earl, C; Wrobel, AG; Benton, DJ; Roustan, C; Bolland, W; Thompson, R; Agua-Doce, A; Hobson, P; Heaney, J; Rickman, H; Paraskevopoulou, S; Houlihan, CF; Thomson, K; Sanchez, E; Shin, GY; Spyer, MJ; Joshi, D; O'Reilly, N; Walker, PA; Kjaer, S; Riddell, A; Moore, C; Jebson, BR; Wilkinson, M; Marshall, LR; Rosser, EC; Radziszewska, A; Peckham, H; Ciurtin, C; Wedderburn, LR; Beale, R; Swanton, C; Gandhi, S; Stockinger, B; McCauley, J; Gamblin, SJ; McCoy, LE; Cherepanov, P; Nastouli, E; Kassiotis, G; - view fewer <#> (2020) Preexisting and de novo humoral immunity to SARS-CoV-2 in humans. Science , Article eabe1107. 10.1126/science.abe1107 <https://doi.org/10.1126/science.abe1107>. (In press). Green open access document_url: https://discovery-pp.ucl.ac.uk/id/eprint/10114820/1/Ng%20et%20al2020Article_Pre-Edit%205.pdf document_url: https://discovery-pp.ucl.ac.uk/id/eprint/10114820/7/Ng%20et%20alScience%202020_Pre-Edit%202.3Suppl.pdf