TY  - INPR
N2  - We recently described aberrantly increased cytoplasmic SFPQ intron-retaining transcripts (IRTs) and concurrent SFPQ protein mislocalization as new hallmarks of amyotrophic lateral sclerosis (ALS). However the generalizability and potential roles of cytoplasmic IRTs in health and disease remain unclear. Here, using time-resolved deep-sequencing of nuclear and cytoplasmic fractions of hiPSCs undergoing motor neurogenesis, we reveal that ALS-causing VCP gene mutations lead to compartment-specific aberrant accumulation of IRTs. Specifically, we identify >100 IRTs with increased cytoplasmic abundance in ALS samples. Furthermore, these aberrant cytoplasmic IRTs possess sequence-specific attributes and differential predicted binding affinity to RNA binding proteins (RBPs). Remarkably, TDP-43, SFPQ and FUS?RBPs known for nuclear-to-cytoplasmic mislocalization in ALS?abundantly and specifically bind to this aberrant cytoplasmic pool of IRTs, as opposed to any individual IRT. Our data are therefore consistent with a novel role for cytoplasmic IRTs in regulating compartment-specific protein abundance. This study provides new molecular insight into potential pathomechanisms underlying ALS and highlights aberrant cytoplasmic IRTs as potential therapeutic targets.
AV  - public
KW  - Cytoplasmic intron retention
KW  -  amyotrophic lateral sclerosis
KW  -  human stem cell model
KW  -  nuclear/cytoplasmic fractionation
KW  -  protein mislocalization
TI  - Aberrant cytoplasmic intron retention is a blueprint for RNA binding protein mislocalization in amyotrophic lateral sclerosis
N1  - Copyright © The Author(s) (2021). Published by Oxford University Press on behalf of the Guarantors of Brain. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
UR  - https://doi.org/10.1093/brain/awab078
A1  - Tyzack, GE
A1  - Neeves, J
A1  - Crerar, H
A1  - Klein, P
A1  - Ziff, O
A1  - Taha, DM
A1  - Luisier, R
A1  - Luscombe, NM
A1  - Patani, R
ID  - discovery10124282
JF  - Brain
Y1  - 2021/03/09/
ER  -