eprintid: 10126373 rev_number: 21 eprint_status: archive userid: 608 dir: disk0/10/12/63/73 datestamp: 2021-04-22 13:24:31 lastmod: 2022-01-07 23:45:57 status_changed: 2021-04-22 13:24:31 type: article metadata_visibility: show creators_name: Kohli, N creators_name: Sharma, V creators_name: Orera, A creators_name: Sawadkar, P creators_name: Owji, N creators_name: Frost, OG creators_name: Bailey, RJ creators_name: Snow, M creators_name: Knowles, JC creators_name: Blunn, GW creators_name: García-Gareta, E title: Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering ispublished: inpress divisions: UCL divisions: B02 divisions: C10 divisions: D21 divisions: H10 divisions: D13 divisions: G22 keywords: Bone tissue engineering, angiogenic, osteogenic, fibrin, calcium phosphate note: This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). abstract: Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation. date: 2021-04-06 date_type: published publisher: SAGE Publications official_url: https://doi.org/10.1177%2F20417314211005610 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1858583 doi: 10.1177/20417314211005610 lyricists_name: Garcia, Elena lyricists_name: Knowles, Jonathan lyricists_name: Owji, Nazanin lyricists_id: EGARC71 lyricists_id: JCKNO52 lyricists_id: NOWJI02 actors_name: Flynn, Bernadette actors_id: BFFLY94 actors_role: owner full_text_status: public publication: Journal of Tissue Engineering volume: 12 citation: Kohli, N; Sharma, V; Orera, A; Sawadkar, P; Owji, N; Frost, OG; Bailey, RJ; ... García-Gareta, E; + view all <#> Kohli, N; Sharma, V; Orera, A; Sawadkar, P; Owji, N; Frost, OG; Bailey, RJ; Snow, M; Knowles, JC; Blunn, GW; García-Gareta, E; - view fewer <#> (2021) Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering. Journal of Tissue Engineering , 12 10.1177/20417314211005610 <https://doi.org/10.1177/20417314211005610>. (In press). Green open access document_url: https://discovery-pp.ucl.ac.uk/id/eprint/10126373/1/20417314211005610.pdf