eprintid: 10189805
rev_number: 13
eprint_status: archive
userid: 699
dir: disk0/10/18/98/05
datestamp: 2024-03-27 08:55:32
lastmod: 2024-03-27 08:55:32
status_changed: 2024-03-27 08:55:32
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Van der Ham, Stijn
creators_name: Agudo-Canalejo, Jaime
creators_name: Vutukuri, Hanumantha Rao
title: Role of Shape in Particle-Lipid Membrane Interactions: From Surfing to Full Engulfment
ispublished: inpress
divisions: UCL
divisions: B04
divisions: C06
divisions: F60
keywords: Vesicles; Lipid membranes; Anisotropic particles; Wrapping; Passive engulfment; Cellular particle uptake
note: This article is licensed under CC-BY 4.0, https://creativecommons.org/licenses/by/4.0/.
abstract: Understanding and manipulating the interactions between foreign bodies and cell membranes during endo- and phagocytosis is of paramount importance, not only for the fate of living cells but also for numerous biomedical applications. This study aims to elucidate the role of variables such as anisotropic particle shape, curvature, orientation, membrane tension, and adhesive strength in this essential process using a minimal experimental biomimetic system comprising giant unilamellar vesicles and rod-like particles with different curvatures and aspect ratios. We find that the particle wrapping process is dictated by the balance between the elastic free energy penalty and adhesion free energy gain, leading to two distinct engulfment pathways, tip-first and side-first, emphasizing the significance of the particle orientation in determining the pathway. Moreover, our experimental results are consistent with theoretical predictions in a state diagram, showcasing how to control the wrapping pathway from surfing to partial to complete wrapping by the interplay between membrane tension and adhesive strength. At moderate particle concentrations, we observed the formation of rod clusters, which exhibited cooperative and sequential wrapping. Our study contributes to a comprehensive understanding of the mechanistic intricacies of endocytosis by highlighting how the interplay between the anisotropic particle shape, curvature, orientation, membrane tension, and adhesive strength can influence the engulfment pathway.
date: 2024-03-21
date_type: published
publisher: American Chemical Society (ACS)
official_url: http://dx.doi.org/10.1021/acsnano.3c11106
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2262511
doi: 10.1021/acsnano.3c11106
medium: Print-Electronic
lyricists_name: Agudo-Canalejo, Jaime
lyricists_id: JAGUD28
actors_name: Agudo-Canalejo, Jaime
actors_id: JAGUD28
actors_role: owner
funding_acknowledgements: [Dutch Research Council (NWO)]
full_text_status: public
publication: ACS Nano
event_location: United States
issn: 1936-0851
citation:        Van der Ham, Stijn;    Agudo-Canalejo, Jaime;    Vutukuri, Hanumantha Rao;      (2024)    Role of Shape in Particle-Lipid Membrane Interactions: From Surfing to Full Engulfment.                   ACS Nano        10.1021/acsnano.3c11106 <https://doi.org/10.1021/acsnano.3c11106>.    (In press).    Green open access   
 
document_url: https://discovery-pp.ucl.ac.uk/id/eprint/10189805/1/Agudo-Canalejo_van-der-ham-et-al-2024-role-of-shape-in-particle-lipid-membrane-interactions-from-surfing-to-full-engulfment.pdf