@article{discovery10192589,
       publisher = {Nature Portfolio},
            note = {This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.},
           month = {February},
           title = {A microfluidic platform integrating functional vascularized organoids-on-chip},
            year = {2024},
          volume = {15},
         journal = {Nature Communications},
        abstract = {The development of vascular networks in microfluidic chips is crucial for the long-term culture of three-dimensional cell aggregates such as spheroids, organoids, tumoroids, or tissue explants. Despite rapid advancement in microvascular network systems and organoid technologies, vascularizing organoids-on-chips remains a challenge in tissue engineering. Most existing microfluidic devices poorly reflect the complexity of in vivo flows and require complex technical set-ups. Considering these constraints, we develop a platform to establish and monitor the formation of endothelial networks around mesenchymal and pancreatic islet spheroids, as well as blood vessel organoids generated from pluripotent stem cells, cultured for up to 30 days on-chip. We show that these networks establish functional connections with the endothelium-rich spheroids and vascular organoids, as they successfully provide intravascular perfusion to these structures. We find that organoid growth, maturation, and function are enhanced when cultured on-chip using our vascularization method. This microphysiological system represents a viable organ-on-chip model to vascularize diverse biological 3D tissues and sets the stage to establish organoid perfusions using advanced microfluidics.},
             url = {https://doi.org/10.1038/s41467-024-45710-4},
            issn = {2041-1723},
        keywords = {Biomedical engineering, Induced pluripotent stem cells, Microfluidics, Stem-cell biotechnology, Tissue engineering},
          author = {Quintard, C and Tubbs, E and Jonsson, G and Jiao, J and Wang, J and Werschler, N and Laporte, C and Pitaval, A and Bah, TS and Pomeranz, G and Bissardon, C and Kaal, J and Leopoldi, A and Long, DA and Blandin, P and Achard, JL and Battail, C and Hagelkruys, A and Navarro, F and Fouillet, Y and Penninger, JM and Gidrol, X}
}