eprintid: 1469916
rev_number: 41
eprint_status: archive
userid: 608
dir: disk0/01/46/99/16
datestamp: 2015-10-15 09:34:09
lastmod: 2021-07-28 00:04:16
status_changed: 2015-10-15 09:34:09
type: thesis
metadata_visibility: show
item_issues_count: 0
creators_name: Harrison, CJ
title: The role of rare codons in protein expression
ispublished: unpub
divisions: UCL
divisions: A01
divisions: B04
divisions: C06
abstract: That the flow of information from gene sequence to protein sequence depends on the translation of a code that could literally be described as digital is a truly incredible feat of nature. However, the process of translation is a noisy, stochastic, kinetic process that depends on many factors. The redundancy in the genetic code allows the transmission of additional, analogue information by varying some of these factors. How organisms use the redundancy is termed codon usage, and rare codons are those that are typically shunned in favour of other synonymous options. Synonymous variations to the codon usage pattern of a gene have been linked to disease, and can have huge effects on the functionality and quantity of protein produced from a gene, but the nature of these variations is complex and poorly understood. In some cases, rare codons appear to have a beneficial influence on expression. This thesis investigates the phenomenon of rare codons and attempts to elucidate their evolutionary role in optimal gene expression. It begins with the design of a novel statistical algorithm, which is used to generate a dataset of interesting genetic locations. The dataset is the subject of a hypothesis-driven investigation to discover meaningful biological correlates, and this is complemented by experimental work, to attempt to provide conclusive validation of the approach.
date: 2015-07-28
vfaculties: VMPS
oa_status: green
full_text_type: other
thesis_class: doctoral_open
language: eng
thesis_view: UCL_Thesis
primo: open
primo_central: open_green
verified: verified_manual
elements_source: Manually entered
elements_id: 1042353
lyricists_name: Harrison, Charlie
lyricists_id: CJHAR91
full_text_status: public
pages: 198
institution: UCL (University College London)
department: CoMPLEX
thesis_type: Doctoral
citation:        Harrison, CJ;      (2015)    The role of rare codons in protein expression.                   Doctoral thesis , UCL (University College London).     Green open access   
 
document_url: https://discovery-pp.ucl.ac.uk/id/eprint/1469916/2/CHarrison_thesis-FINAL.pdf