eprintid: 1470016
rev_number: 42
eprint_status: archive
userid: 608
dir: disk0/01/47/00/16
datestamp: 2015-11-04 12:25:58
lastmod: 2021-10-04 01:41:28
status_changed: 2015-11-04 12:25:58
type: article
metadata_visibility: show
creators_name: Patil, SB
creators_name: Vögtli, M
creators_name: Webb, B
creators_name: Mazza, G
creators_name: Pinzani, M
creators_name: Soh, YA
creators_name: McKendry, RA
creators_name: Ndieyira, JW
title: Decoupling competing surface binding kinetics and reconfiguration of receptor footprint for ultrasensitive stress assays
ispublished: pub
divisions: UCL
divisions: B02
divisions: C10
divisions: D17
divisions: G94
divisions: G91
divisions: B04
divisions: C06
divisions: F64
abstract: Cantilever arrays have been used to monitor biochemical interactions and their associated stress. However, it is often necessary to passivate the underside of the cantilever to prevent unwanted ligand adsorption, and this process requires tedious optimization. Here, we show a way to immobilize membrane receptors on nanomechanical cantilevers so that they can function without passivating the underlying surface. Using equilibrium theory, we quantitatively describe the mechanical responses of vancomycin, human immunodeficiency virus type 1 antigens and coagulation factor VIII captured on the cantilever in the presence of competing stresses from the top and bottom cantilever surfaces. We show that the area per receptor molecule on the cantilever surface influences ligand-receptor binding and plays an important role on stress. Our results offer a new way to sense biomolecules and will aid in the creation of ultrasensitive biosensors.
date: 2015-08-17
official_url: http://dx.doi.org/10.1038/nnano.2015.174
vfaculties: VMPS
vfaculties: VMPS
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1043552
doi: 10.1038/nnano.2015.174
pii: nnano.2015.174
lyricists_name: Mazza, Giuseppe
lyricists_name: McKendry, Rachel
lyricists_name: Ndieyira, Joseph
lyricists_name: Pinzani, Massimo
lyricists_id: GMAZZ02
lyricists_id: RMCKE57
lyricists_id: JWNDI09
lyricists_id: MPINZ74
actors_name: Ndieyira, Joseph
actors_id: JWNDI09
actors_role: owner
full_text_status: public
publication: Nature Nanotechnology
volume: 10
number: 10
pagerange: 899-907
issn: 1748-3395
citation:        Patil, SB;    Vögtli, M;    Webb, B;    Mazza, G;    Pinzani, M;    Soh, YA;    McKendry, RA;           Patil, SB;  Vögtli, M;  Webb, B;  Mazza, G;  Pinzani, M;  Soh, YA;  McKendry, RA;  Ndieyira, JW;   - view fewer <#>    (2015)    Decoupling competing surface binding kinetics and reconfiguration of receptor footprint for ultrasensitive stress assays.                   Nature Nanotechnology , 10  (10)   pp. 899-907.    10.1038/nnano.2015.174 <https://doi.org/10.1038/nnano.2015.174>.       Green open access   
 
document_url: https://discovery-pp.ucl.ac.uk/id/eprint/1470016/3/NNANO-14112052A-Ndieyira-MS_Revised_Final_template.pdf