eprintid: 1472547 rev_number: 26 eprint_status: archive userid: 608 dir: disk0/01/47/25/47 datestamp: 2015-11-13 15:44:49 lastmod: 2021-12-13 02:08:13 status_changed: 2015-11-13 15:44:49 type: article metadata_visibility: show creators_name: He, Q creators_name: Duguid, I creators_name: Clark, B creators_name: Panzanelli, P creators_name: Patel, B creators_name: Thomas, P creators_name: Fritschy, JM creators_name: Smart, TG title: Interneuron- and GABA(A) receptor-specific inhibitory synaptic plasticity in cerebellar Purkinje cells ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: G02 divisions: C10 divisions: D17 divisions: G95 note: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ abstract: Inhibitory synaptic plasticity is important for shaping both neuronal excitability and network activity. Here we investigate the input and GABA(A) receptor subunit specificity of inhibitory synaptic plasticity by studying cerebellar interneuron-Purkinje cell (PC) synapses. Depolarizing PCs initiated a long-lasting increase in GABA-mediated synaptic currents. By stimulating individual interneurons, this plasticity was observed at somatodendritic basket cell synapses, but not at distal dendritic stellate cell synapses. Basket cell synapses predominantly express β2-subunit-containing GABA(A) receptors; deletion of the β2-subunit ablates this plasticity, demonstrating its reliance on GABA(A) receptor subunit composition. The increase in synaptic currents is dependent upon an increase in newly synthesized cell surface synaptic GABA(A) receptors and is abolished by preventing CaMKII phosphorylation of GABA(A) receptors. Our results reveal a novel GABA(A) receptor subunit- and input-specific form of inhibitory synaptic plasticity that regulates the temporal firing pattern of the principal output cells of the cerebellum. date: 2015 date_type: published official_url: http://dx.doi.org/10.1038/ncomms8364 oa_status: green full_text_type: pub pmcid: PMC4518301 primo: open primo_central: open_green article_type_text: Journal Article verified: verified_manual elements_id: 1043828 doi: 10.1038/ncomms8364 pii: ncomms8364 language_elements: eng lyricists_name: Clark, Beverley lyricists_name: Smart, Trevor lyricists_name: Thomas, Philip lyricists_id: BACLA52 lyricists_id: TSMAR12 lyricists_id: PTHOM42 actors_name: Clark, Beverley actors_name: Poirier, Elizabeth actors_id: BACLA52 actors_id: EPPOI23 actors_role: owner actors_role: impersonator full_text_status: public publication: Nat Commun volume: 6 article_number: 7364 event_location: England issn: 2041-1723 citation: He, Q; Duguid, I; Clark, B; Panzanelli, P; Patel, B; Thomas, P; Fritschy, JM; He, Q; Duguid, I; Clark, B; Panzanelli, P; Patel, B; Thomas, P; Fritschy, JM; Smart, TG; - view fewer <#> (2015) Interneuron- and GABA(A) receptor-specific inhibitory synaptic plasticity in cerebellar Purkinje cells. Nat Commun , 6 , Article 7364. 10.1038/ncomms8364 <https://doi.org/10.1038/ncomms8364>. Green open access document_url: https://discovery-pp.ucl.ac.uk/id/eprint/1472547/1/Interneuron-%20and%20GABA%28A%29%20receptor-specific%20inhibitory%20synaptic%20plasticity%20in%20cerebellar%20Purkinje%20cells..pdf