eprintid: 1549535
rev_number: 34
eprint_status: archive
userid: 608
dir: disk0/01/54/95/35
datestamp: 2017-04-09 16:35:57
lastmod: 2021-09-19 22:16:33
status_changed: 2017-06-28 15:41:28
type: article
metadata_visibility: show
creators_name: Ward, DP
creators_name: Hewitson, P
creators_name: Cardenas-Fernandez, M
creators_name: Hamley-Bennett, C
creators_name: Diaz-Rodriguez, A
creators_name: Douillet, N
creators_name: Adams, JP
creators_name: Leak, DJ
creators_name: Ignatova, S
creators_name: Lye, GJ
title: Centrifugal partition chromatography in a biorefinery context: Optimisation and scale-up of monosaccharide fractionation from hydrolysed sugar beet pulp
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: F47
keywords: Monosaccharides;
    Sugar beet pulp;
    Biorefinery;
    Scale-up;
    Centrifugal partition chromatography
note: Copyright © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license(http://creativecommons.org/licenses/by/4.0/).
abstract: The isolation of component sugars from biomass represents an important step in the bioprocessing of sustainable feedstocks such as sugar beet pulp. Centrifugal partition chromatography (CPC) is used here, as an alternative to multiple resin chromatography steps, to fractionate component monosaccharides from crude hydrolysed sugar beet pulp pectin. CPC separation of samples, prepared in the stationary phase, was carried out using an ethanol: ammonium sulphate (300 g L−1) phase system (0.8:1.8 v:v) in ascending mode. This enabled removal of crude feedstream impurities and separation of monosaccharides into three fractions (l-rhamnose, l-arabinose and d-galactose, and d-galacturonic acid) in a single step. Throughput was improved three-fold by increasing sample injection volume, from 4 to 16% of column volume, with similar separation performance maintained in all cases. Extrusion of the final galacturonic acid fraction increased the eluted solute concentration, reduced the total separation time by 24% and removed the need for further column regeneration. Reproducibility of the separation after extrusion was validated by using multiple stacked injections. Scale-up was performed linearly from a semi-preparative 250 mL column to a preparative 950 mL column with a scale-up ratio of 3.8 applied to mobile phase flow rate and sample injection volume. Throughputs of 9.4 g L−1 h−1 of total dissolved solids were achieved at the preparative scale with a throughput of 1.9 g L−1 h−1 of component monosaccharides. These results demonstrate the potential of CPC for both impurity removal and target fractionation within biorefinery separations.
date: 2017-05-12
date_type: published
publisher: ELSEVIER SCIENCE BV
official_url: https://doi.org/10.1016/j.chroma.2017.03.003
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1285066
doi: 10.1016/j.chroma.2017.03.003
lyricists_name: Cardenas-Fernandez, Anthony
lyricists_name: Lye, Gary
lyricists_id: AMCAR49
lyricists_id: GJLYE60
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Journal of Chromatography A
volume: 1497
pagerange: 56-63
pages: 8
event_location: Chicago, IL
issn: 1873-3778
citation:        Ward, DP;    Hewitson, P;    Cardenas-Fernandez, M;    Hamley-Bennett, C;    Diaz-Rodriguez, A;    Douillet, N;    Adams, JP;             ... Lye, GJ; + view all <#>        Ward, DP;  Hewitson, P;  Cardenas-Fernandez, M;  Hamley-Bennett, C;  Diaz-Rodriguez, A;  Douillet, N;  Adams, JP;  Leak, DJ;  Ignatova, S;  Lye, GJ;   - view fewer <#>    (2017)    Centrifugal partition chromatography in a biorefinery context: Optimisation and scale-up of monosaccharide fractionation from hydrolysed sugar beet pulp.                   Journal of Chromatography A , 1497    pp. 56-63.    10.1016/j.chroma.2017.03.003 <https://doi.org/10.1016/j.chroma.2017.03.003>.       Green open access   
 
document_url: https://discovery-pp.ucl.ac.uk/id/eprint/1549535/1/Ward_Centrifugal_partition_chromatography.pdf