eprintid: 1570085
rev_number: 40
eprint_status: archive
userid: 608
dir: disk0/01/57/00/85
datestamp: 2017-08-13 03:46:15
lastmod: 2021-10-03 23:49:24
status_changed: 2017-11-03 12:11:45
type: article
metadata_visibility: show
creators_name: Kopach, O
creators_name: Krotov, V
creators_name: Voitenko, N
title: Atlanto-occipital catheterization of young rats for long-term drug delivery into the lumbar subarachnoid space combined with in vivo testing and electrophysiology in situ
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F81
divisions: C08
divisions: D09
divisions: G02
keywords: Behavioral testing, Genetic material, Intrathecal delivery, Local treatment, Oligodeoxynucleotides, Peripheral sensitivity, Sensory interneurons, Spinal catheterization, Spinal cord electrophysiology, Young animals
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: BACKGROUND: Catheterization has been widely used in neuroscience and pain research for local drug delivery. Though different modifications were developed, the use of young animals for spinal catheterization remains limited because of a little success rate. A reliable technique is needed to catheterize young animals aimed for in vivo testing combined with spinal cord electrophysiology, often limited by animal age, to facilitate pain research. NEW METHODS: We describe intrathecal catheterization of young rats (3-week-old) through atlanto-occipical approach for long-lasting drug delivery into the lumbar subarachnoid space. The technique represents a surgical approach of minimized invasiveness that requires PE-10 catheter and few equipment of standard laboratory use. RESULTS: Behavioral assessments revealed that spinal catheterization does not change peripheral sensitivity of different modalities (thermal and mechanical) and gives no rise to locomotive deficit or anxiety-like behavior in young rats. The long-term administration of genetic material (oligodeoxynucleotides given up to 4days), examined both in vivo and in situ, produced no adverse effects on basal peripheral sensitivity, but changed the AMPA receptor-mediated currents in sensory interneurons of the spinal cord. COMPARISON WITH EXISTING METHODS: Dissimilar to already described methods, the method is designed for the use of young rats for behavioral testing in vivo and/or spinal cord electrophysiology in situ. CONCLUSIONS: A practical method for spinal catheterization of young animals designed for studies in vivo and in situ is proposed. The method is rapid and effective and should facilitate investigation of therapeutic effects on both systemic and subcellular levels, as an advantage over the existing methods.
date: 2017-10
date_type: published
official_url: http://doi.org/10.1016/j.jneumeth.2017.08.001
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1413144
doi: 10.1016/j.jneumeth.2017.08.001
pii: S0165-0270(17)30276-5
lyricists_name: Kopach, Olga
lyricists_name: Krotov, Volodymyr
lyricists_id: OKOPA85
lyricists_id: VKROT77
full_text_status: public
publication: Journal of Neuroscience Methods
volume: 290
pagerange: 125-132
event_location: Netherlands
issn: 1872-678X
citation:        Kopach, O;    Krotov, V;    Voitenko, N;      (2017)    Atlanto-occipital catheterization of young rats for long-term drug delivery into the lumbar subarachnoid space combined with in vivo testing and electrophysiology in situ.                   Journal of Neuroscience Methods , 290    pp. 125-132.    10.1016/j.jneumeth.2017.08.001 <https://doi.org/10.1016/j.jneumeth.2017.08.001>.       Green open access   
 
document_url: https://discovery-pp.ucl.ac.uk/id/eprint/1570085/1/Kopach_accepted%20MS.pdf