TY  - JOUR
Y1  - 2017/09//
ID  - discovery1574534
JF  - Physiological Reports
SN  - 2051-817X
A1  - Beikoghli Kalkhoran, S
A1  - Hall, AR
A1  - White, IJ
A1  - Cooper, J
A1  - Fan, Q
A1  - Ong, S-B
A1  - Hernández-Reséndiz, S
A1  - Cabrera-Fuentes, H
A1  - Chinda, K
A1  - Chakraborty, B
A1  - Dorn, GW
A1  - Yellon, DM
A1  - Hausenloy, DJ
UR  - http://dx.doi.org/10.14814/phy2.13437
N1  - © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
TI  - Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography
N2  - The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria-jSR complex in the adult heart.
IS  - 17
AV  - public
VL  - 5
KW  - 3D electron tomography
KW  -  MFN2 KO
KW  -  mitochondria?jSR junction
ER  -