Eisenberg-Bord, M;
Mari, M;
Weill, U;
Rosenfeld-Gur, E;
Moldavski, O;
Castro, IG;
Soni, KG;
... Bohnert, M; + view all
(2017)
Identification of seipin-linked factors that act as determinants of a lipid droplet subpopulation.
Journal of Cell Biology
, 2018
pp. 1-14.
10.1083/jcb.201704122.
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Abstract
Functional heterogeneity within the lipid droplet (LD) pool of a single cell has been observed, yet the underlying mechanisms remain enigmatic. Here, we report on identification of a specialized LD subpopulation characterized by a unique proteome and a defined geographical location at the nucleus-vacuole junction contact site. In search for factors determining identity of these LDs, we screened ∼6,000 yeast mutants for loss of targeting of the subpopulation marker Pdr16 and identified Ldo45 (LD organization protein of 45 kD) as a crucial targeting determinant. Ldo45 is the product of a splicing event connecting two adjacent genes (YMR147W and YMR148W/OSW5/LDO16). We show that Ldo proteins cooperate with the LD biogenesis component seipin and establish LD identity by defining positioning and surface-protein composition. Our studies suggest a mechanism to establish functional differentiation of organelles, opening the door to better understanding of metabolic decisions in cells.
| Type: | Article |
|---|---|
| Title: | Identification of seipin-linked factors that act as determinants of a lipid droplet subpopulation |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1083/jcb.201704122 |
| Publisher version: | https://doi.org/10.1083/jcb.201704122 |
| Language: | English |
| Additional information: | This article is available under a Creative Commons License (Attribution 4.0 International, as described at https ://creativecommons .org /licenses /by /4 .0 /) |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10039596 |
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