Freire-Pritchett, P;
Schoenfelder, S;
Várnai, C;
Wingett, SW;
Cairns, J;
Collier, AJ;
García-Vílchez, R;
... Spivakov, M; + view all
(2017)
Global reorganisation of cis-regulatory units upon lineage commitment of human embryonic stem cells.
eLIFE
, 6
, Article e21926. 10.7554/eLife.21926.
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Abstract
Long-range cis-regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of cis-regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving ~22,000 gene promoters in human pluripotent and lineage-committed cells, identifying putative target genes for known and predicted enhancer elements. We reveal extensive dynamics of cis-regulatory contacts upon lineage commitment, including the acquisition and loss of promoter interactions. This spatial rewiring occurs preferentially with predicted changes in the activity of cis-regulatory elements and is associated with changes in target gene expression. Our results provide a global and integrated view of promoter interactome dynamics during lineage commitment of human pluripotent cells.
| Type: | Article |
|---|---|
| Title: | Global reorganisation of cis-regulatory units upon lineage commitment of human embryonic stem cells |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.7554/eLife.21926 |
| Publisher version: | https://doi.org/10.7554/eLife.21926.001 |
| Language: | English |
| Additional information: | © 2017, Freire-Pritchett et al. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | DNA, cis-regulatory elements, Promoter Capture Hi-C, Human embryonic stem cells |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10047223 |
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