Chetty, CJ;
Ferreira, E;
Jovanovic, K;
Weiss, SFT;
(2017)
Knockdown of LRP/LR induces apoptosis in pancreatic cancer and neuroblastoma cells through activation of caspases.
Experimental Cell Research
, 360
(2)
pp. 264-272.
10.1016/j.yexcr.2017.09.016.
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Abstract
The 37 kDa/67 kDa laminin receptor (LRP/LR) serves various physiological and pathological roles such as enhancing tumour-related processes including metastasis, angiogenesis, cellular viability and telomerase activation in cancerous cell lines. The present study investigates the effect of siRNA mediated downregulation of LRP/LR on pancreatic cancer (AsPC-1) and neuroblastoma (IMR-32) cells. MTT and BrdU assays revealed that siRNA mediated downregulation of LRP resulted in a significant reduction in cell viability and cell proliferation. In addition, knock-down of LRP resulted in phosphatidylserine externalization, diminished nuclear integrity and significantly enhanced caspase-3 activity, which is indicative of apoptosis. LRP downregulation resulted in a significant increase in caspase-8 activity in IMR-32 cells and enhanced caspase-8 and 9 activity in AsPC-1 cells. These data recommend siRNA mediated knock-down of LRP as a potential therapeutic avenue for the treatment of pancreatic cancer and neuroblastoma.
| Type: | Article |
|---|---|
| Title: | Knockdown of LRP/LR induces apoptosis in pancreatic cancer and neuroblastoma cells through activation of caspases |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.yexcr.2017.09.016 |
| Publisher version: | http://dx.doi.org/10.1016/j.yexcr.2017.09.016 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Oncology, Cell Biology, 37 kDa/67 kDa laminin Receptor, Apoptosis, Small interfering FtNAs, Pancreatic cancer, Neuroblastoma, KDA LAMININ RECEPTOR, SIGNAL-TRANSDUCTION, IGG1-IS18 IMPEDES, NEURONAL CELLS, INVASION, ADHESION, METASTASIS, MOLECULES, PRECURSOR, PROTEINS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10047388 |
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