Luo, B;
Wu, S;
Zhang, Z;
Zou, W;
Shi, S;
Zhao, M;
Zhong, N;
... Zhang, L; + view all
(2017)
Human heart failure biomarker immunosensor based on excessively tilted fiber gratings.
Biomedical Optics Express
, 8
(1)
pp. 57-67.
10.1364/BOE.8.000057.
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Abstract
A label-free immunosensor platform based on excessively tilted fiber gratings (Ex-TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NT-proBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NT-proBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
| Type: | Article |
|---|---|
| Title: | Human heart failure biomarker immunosensor based on excessively tilted fiber gratings |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1364/BOE.8.000057 |
| Publisher version: | https://doi.org/10.1364/BOE.8.000057 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Biological sensing and sensors, Fiber optics sensors, Medical optics and biotechnology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10049734 |
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