Kestemont, D;
Renders, M;
Leonczak, P;
Abramov, M;
Schepers, G;
Pinheiro, VB;
Rozenski, J;
(2018)
XNA ligation using T4 DNA ligase in crowding conditions.
Chemical Communications
, 54
(49)
pp. 6408-6411.
10.1039/C8CC02414F.
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Abstract
T4 DNA ligase is capable of ligating 2′OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.
| Type: | Article |
|---|---|
| Title: | XNA ligation using T4 DNA ligase in crowding conditions |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/C8CC02414F |
| Publisher version: | https://doi.org/10.1039/C8CC02414F |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10049874 |
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