Reardon, TR;
Murray, AJ;
Turi, GF;
Wirblich, C;
Croce, KR;
Schnell, MJ;
Jessell, TM;
(2016)
Rabies Virus CVS-N2c(Delta G) Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability.
Neuron
, 89
(4)
pp. 711-724.
10.1016/j.neuron.2016.01.004.
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Rabies virus CVS-N2cΔG strain enhances retrograde synaptic transfer and neuronal viability.pdf - Accepted Version Download (2MB) | Preview |
Abstract
Summary Virally based transsynaptic tracing technologies are powerful experimental tools for neuronal circuit mapping. The glycoprotein-deletion variant of the SAD-B19 vaccine strain rabies virus (RABV) has been the reagent of choice in monosynaptic tracing, since it permits the mapping of synaptic inputs to genetically marked neurons. Since its introduction, new helper viruses and reagents that facilitate complementation have enhanced the efficiency of SAD-B19ΔG transsynaptic transfer, but there has been little focus on improvements to the core RABV strain. Here we generate a new deletion mutant strain, CVS-N2cΔG, and examine its neuronal toxicity and efficiency in directing retrograde transsynaptic transfer. We find that by comparison with SAD-B19ΔG, the CVS-N2cΔG strain exhibits a reduction in neuronal toxicity and a marked enhancement in transsynaptic neuronal transfer. We conclude that the CVS-N2cΔG strain provides a more effective means of mapping neuronal circuitry and of monitoring and manipulating neuronal activity in vivo in the mammalian CNS.
| Type: | Article |
|---|---|
| Title: | Rabies Virus CVS-N2c(Delta G) Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.neuron.2016.01.004 |
| Publisher version: | https://doi.org/10.1016/j.neuron.2016.01.004 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Neurosciences, Neurosciences & Neurology, CENTRAL-NERVOUS-SYSTEM, TRANSNEURONAL TRACER, DENDRITIC INHIBITION, GENE-EXPRESSION, OPTICAL CONTROL, BASAL GANGLIA, GLYCOPROTEIN, CIRCUIT, CONNECTIVITY, PATHOGENESIS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > The Sainsbury Wellcome Centre |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10051299 |
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