Corbett, AD;
Shaw, M;
Yacoot, A;
Jefferson, A;
Schermelleh, L;
Wilson, T;
Booth, M;
(2018)
Microscope calibration using laser written fluorescence.
Optics Express
, 26
(17)
pp. 21887-21899.
10.1364/OE.26.021887.
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Abstract
There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and color plane alignment using confocal and structured illumination fluorescence microscopes.
| Type: | Article |
|---|---|
| Title: | Microscope calibration using laser written fluorescence |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1364/OE.26.021887 |
| Publisher version: | https://doi.org/10.1364/OE.26.021887 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10054957 |
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