UCL Discovery Stage
UCL home » Library Services » Electronic resources » UCL Discovery Stage

NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy

Tian, X; Liu, T; Fang, B; Wang, A; Zhang, M; Hussain, S; Luo, L; ... Tian, Y; + view all (2018) NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy. ACS Applied Materials & Interfaces , 10 (38) pp. 31959-31964. 10.1021/acsami.8b11102. Green open access

[thumbnail of art-template-1.7.pdf]
Preview
Text
art-template-1.7.pdf - Accepted Version

Download (13MB) | Preview

Abstract

Neuronal nuclei (NeuN) protein is a key RNA-associated protein to guide the transcription of RNA and regulate mRNA splicing in neurons. However, the lack of effective labeling and tracking method has hindered the elucidation of the biological mechanism of NeuN operation in living neurons to understand correlated central nervous system disorders. Here, we report a molecular probe that can be inserted into a neighboring hydrophobic-hydrophilic region in NeuN, which upon binding becomes capable of emitting light in red region. The NeuN specificity enables the probe imaging neuronal cells in primary brain regions including hippocampus, cerebellum, midbrain, and cingulate gyrus. The probes' optical properties are such to enable stimulated emission depletion imaging showing for the first time the 3D structure of RNA tangling into NeuN in a living neuron with tens of nanometer resolution.

Type: Article
Title: NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acsami.8b11102
Publisher version: https://doi.org/10.1021/acsami.8b11102
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: STED nanoscopy, fluorescent probe, living neurons, neuronal nuclei protein
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10055330
Downloads since deposit
13,080Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item