Mollenhauer, B;
DuBois, BF;
Drake, D;
Duong, J;
Blennow, K;
El-Agnaf, O;
Shaw, LM;
... Zetterberg, H; + view all
(2019)
Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study.
Journal of Neurochemistry
, 149
(1)
pp. 126-138.
10.1111/jnc.14569.
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Antibody-based methods for the measurement of α-synuclein concentration in human cerebrospinal fluid - method comparison and.pdf - Published Version Download (2MB) | Preview |
Abstract
α-Synuclein is the major component of Lewy bodies (LBs) and a candidate biomarker for neurodegenerative diseases in which LBs are common, including Parkinson's disease and dementia with Lewy bodies. A large body of literature suggests that these disorders are characterised by reduced concentrations of α-synuclein in cerebrospinal fluid (CSF), with overlapping concentrations compared to healthy controls and variability across studies. Several reasons can account for this variability, including technical ones, such as inter-assay and inter-laboratory variation (reproducibility). We compared four (4) immunochemical methods for the quantification of α-synuclein concentration in 50 unique CSF samples. All methods were designed to capture most of the existing α-synuclein forms in CSF ("total" α-synuclein). Each of the four methods showed high analytical precision, excellent correlation between laboratories (R2 0.83-0.99) and good correlation with each other (R2 0.64-0.93), although the slopes of the regression lines were different between the four immunoassays. The use of common reference CSF samples decreased the differences in α-synuclein concentration between detection methods and technologies. Pilot data on an immunoprecipitation mass spectrometry (IP-MS) method is also presented. Our results suggest that the four immunochemical methods and the IP-MS method measure similar forms of α-synuclein and that a common reference material would allow harmonization of results between immunoassays. This article is protected by copyright. All rights reserved.
| Type: | Article |
|---|---|
| Title: | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/jnc.14569 |
| Publisher version: | https://doi.org/10.1111/jnc.14569 |
| Language: | English |
| Additional information: | Copyright © 2018 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | biomarker, cerebrospinal fluid, enzyme-linked immunoabsorbent assay, mass spectrometry, round robin, α-synuclein |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10056552 |
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