Jaiswal, N;
Pandey, CM;
Soni, A;
Tiwari, I;
Rosillo-Lopez, M;
Salzmann, CG;
Malhotra, BD;
(2018)
Electrochemical genosensor based on carboxylated graphene for detection of water-borne pathogen.
Sensors and Actuators, B: Chemical
, 275
pp. 312-321.
10.1016/j.snb.2018.07.055.
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Abstract
This work reports the application of newly synthesized carboxylated graphene nanoflakes (Cx-Gnfs). The Cx-Gnfs were synthesized by wet chemical method in sulfuric acid/nitric acid mixture and was further electrophoretically deposited on indium tin oxide (ITO) coated glass substrates using Mg2+ions which provides an overall charge to the materials for deposition onto the anode. The materials were characterized using SEM, TEM, contact angle, UV–vis spectroscopy, FT-IR, XRD and electrochemically characterized by cyclic voltammetry, chronocoulometry and electrochemical impedance spectroscopy. The sensitive quantitative determination of nucleic acid Escherichia coli O157: H7 (E. coli) has been achieved using Cx-Gnfs and r-GO as the sensing layer using electrochemical impedance spectroscopy. The electrochemical results reveal that the Cx-Gnfs based genosensor exhibits a linear response to complementary DNA (10−6M to 10−17M) with a detection limit of 1 × 10−17M while the rGO based genosensor shows a detection limit of 1 × 10−15M. Under optimal conditions, this Cx-Gnfs based genosensor was found to retain about 85% of its initial activity after being used for 6 times.
| Type: | Article |
|---|---|
| Title: | Electrochemical genosensor based on carboxylated graphene for detection of water-borne pathogen |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.snb.2018.07.055 |
| Publisher version: | https://doi.org/10.1016/j.snb.2018.07.055 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | E.coli, Graphene nanoflakes, Biosensors, Reduced graphene oxide, Electrophoretic deposition |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10056810 |
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