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Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes

Rey, G; Milev, NB; Valekunja, UK; Ch, R; Ray, S; Silva Dos Santos, M; Nagy, AD; ... Reddy, AB; + view all (2018) Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes. Molecular Systems Biology , 14 (8) , Article e8376. 10.15252/msb.20188376. Green open access

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Abstract

Circadian rhythms are cell‐autonomous biological oscillations with a period of about 24 h. Current models propose that transcriptional feedback loops are the primary mechanism for the generation of circadian oscillations. Within this framework, Drosophila S2 cells are regarded as “non‐rhythmic” cells, as they do not express several canonical circadian components. Using an unbiased multi‐omics approach, we made the surprising discovery that Drosophila S2 cells do in fact display widespread daily rhythms. Transcriptomics and proteomics analyses revealed that hundreds of genes and their products, and in particular metabolic enzymes, are rhythmically expressed in a 24‐h cycle. Metabolomics analyses extended these findings and demonstrate that central carbon metabolism and amino acid metabolism are core metabolic pathways driven by protein rhythms. We thus demonstrate that 24‐h metabolic oscillations, coupled to gene and protein cycles, take place in nucleated cells without the contribution of any known circadian regulators. These results therefore suggest a reconsideration of existing models of the clockwork in Drosophila and other eukaryotic systems.Mol Syst Biol. (2018) 14: e8376

Type: Article
Title: Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes
Open access status: An open access version is available from UCL Discovery
DOI: 10.15252/msb.20188376
Publisher version: http://dx.doi.org/10.15252/msb.20188376
Language: English
Additional information: This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2018 The Authors. Published under the terms of the CC BY 4.0 license
Keywords: Circadian, Clocks, Metabolomics, Proteomics, Transcriptomics
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10056963
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