Toro-Tapia, G;
Villaseca, S;
Beyer, A;
Roycroft, A;
Marcellini, S;
Mayor, R;
Torrejón, M;
(2018)
The Ric-8A/Gα13/FAK signaling cascade controls focal adhesion formation during neural crest cell migration.
Development
, 145
(22)
, Article dev164269. 10.1242/dev.164269.
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Abstract
Ric-8A is a pleiotropic guanine nucleotide exchange factor involved the activation of various heterotrimeric G protein pathways during adulthood and early development. Here, we sought to determine the downstream effectors of Ric-8A during the migration of the vertebrate cranial neural crest (NC) cells. We show that the Gα13 knockdown phenocopies the Ric-8A morphant condition, causing actin cytoskeleton alteration, protrusion instability and a strong reduction in the number and dynamics of focal adhesions. In addition, the overexpression of Gα13 is sufficient to rescue Ric-8A depleted cells. Ric-8A and Gα13 physically interact and co-localize in protrusions of the cells leading edge. The focal adhesion kinase FAK co-localizes and interacts with the endogenous Gα13, and a constitutively active form of Src efficiently rescues the Gα13 morphant phenotype in NC cells. We propose that Ric-8A-mediated Gα13 signaling is required for proper cranial NC cell migration by regulating focal adhesion dynamics and protrusion formation.
| Type: | Article |
|---|---|
| Title: | The Ric-8A/Gα13/FAK signaling cascade controls focal adhesion formation during neural crest cell migration |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1242/dev.164269 |
| Publisher version: | https://doi.org/10.1242/dev.164269 |
| Language: | English |
| Additional information: | This is the published version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Cell migration, Focal adhesion, GEF, Heterotrimeric G-Protein, Neural crest, Protrusion formation, Ric-8A |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10058754 |
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