Giannousi, K;
Hatzivassiliou, E;
Mourdikoudis, S;
Vourlias, G;
Pantazaki, A;
Dendrinou-Samara, C;
(2016)
Synthesis and biological evaluation of PEGylated CuO nanoparticles.
Journal of Inorganic Biochemistry
, 164
pp. 82-90.
10.1016/j.jinorgbio.2016.09.003.
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Abstract
There is a growing field of research into the physicochemical properties of metal oxide nanoparticles (NPs) and their potential use against tumor formation, development and progression. Coated NPs with biocompatible surfactants can be incorporated into the natural metabolic pathway of the body and specifically favor delivery to the targeted cancerous cells versus normal cells. Polyethylene glycol (PEG) is an FDA approved, biocompatible synthetic polymer and PEGylated NPs are regarded as “stealth” nanoparticles, which are not recognized by the immune system. Herein, PEGylated cupric oxide nanoparticles (CuO NPs) with either PEG 1000 or PEG 8000 were hydrothermally prepared upon properly adjusting the reaction conditions. Depending on the reaction time CuO NPs in the range of core sizes 11–20 nm were formed, while hydrodynamic sizes substantially varied (330–1120 nm) with improved colloidal stability in PBS. The anticancer activity of the NPs was evaluated on human cervical carcinoma HeLa cells by using human immortalized embryonic kidney 293 FT cells as a control. Viability assays (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT) revealed that CuO NPs could selectively reduce viability of tumor cells (IC50 values 11.91–25.78 μg/mL). Reactive oxygen species (ROS) production, cell membrane damage and apoptotic DNA laddering were also evident by nitroblue tetrazolium (NBT) reduction, lactate dehydrogenase (LDH) release assays and DNA electrophoresis, respectively. CuO NPs strongly inhibited lipoxygenase (LOX) enzymatic activity with IC50 values 4–5.9 μg/mL, highlighting in that manner their anti-inflammatory activity.
| Type: | Article |
|---|---|
| Title: | Synthesis and biological evaluation of PEGylated CuO nanoparticles |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.jinorgbio.2016.09.003 |
| Publisher version: | http://dx.doi.org/10.1016/j.jinorgbio.2016.09.003 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Chemistry, Inorganic & Nuclear, Chemistry, Copper oxide nanopartides, Anticancer activity, Anti-inflammatory activity, COPPER-BASED NANOPARTICLES, OXIDATIVE STRESS, ENGINEERED NANOPARTICLES, OXIDE NANOPARTICLES, CELLS, MECHANISMS, TOXICITY, GROWTH, CANCER, CU2O |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10060202 |
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