Pathania, M;
Acosta-Gutierrez, S;
Bhamidimarri, SP;
Basle, A;
Winterhalter, M;
Ceccarelli, M;
van den Berg, B;
(2018)
Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae.
Structure
, 26
(5)
pp. 708-721.
10.1016/j.str.2018.03.010.
Preview |
Text
OmpUT-final.pdf - Accepted Version Download (255kB) | Preview |
Abstract
The outer membranes (OM) of many Gram-negative bacteria contain general porins, which form nonspecific, large-diameter channels for the diffusional uptake of small molecules required for cell growth and function. While the porins of Enterobacteriaceae (e.g., E. coli OmpF and OmpC) have been extensively characterized structurally and biochemically, much less is known about their counterparts in Vibrionaceae. Vibrio cholerae, the causative agent of cholera, has two major porins, OmpU and OmpT, for which no structural information is available despite their importance for the bacterium. Here we report high-resolution X-ray crystal structures of V. cholerae OmpU and OmpT complemented with molecular dynamics simulations. While similar overall to other general porins, the channels of OmpU and OmpT have unusual constrictions that create narrower barriers for small-molecule permeation and change the internal electric fields of the channels. Together with electrophysiological and in vitro transport data, our results illuminate small-molecule uptake within the Vibrionaceae.
| Type: | Article |
|---|---|
| Title: | Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.str.2018.03.010 |
| Publisher version: | https://doi.org/10.1016/j.str.2018.03.010 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | outer membrane protein, porin, Vibrio cholerae, crystal structure, OmpU, OmpT, bile salts, electrophysiology, membrane channel |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10061943 |
Archive Staff Only
 |
View Item |
