Menegollo, M;
Tessari, I;
Bubacco, L;
Szabadkai, G;
(2019)
Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells.
In: Raffaello, A and Vecellio Reane, D, (eds.)
Calcium Signalling: Methods and Protocols.
(pp. 223-232).
Springer Nature: New York (NY), USA.
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Abstract
Cytoplasmic and mitochondrial Ca²⁺ signals couple cellular ATP production to activity-related energy demand. In order to accurately determine the bioenergetic effect of Ca²⁺ signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.
| Type: | Book chapter |
|---|---|
| Title: | Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells |
| Location: | United States |
| ISBN-13: | 978-1-4939-9017-7 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-9018-4_19 |
| Publisher version: | https://doi.org/10.1007/978-1-4939-9018-4_19 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Reversed-phase high-performance liquid chromatography (RP-HPLC), ATP/ADP ratio, Energy charge, Glycolysis, Oxidative phosphorylation, Ca²⁺ signal |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10073232 |
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