Natesh, R;
Clare, DK;
Farr, GW;
Horwich, AL;
Saibil, HR;
(2018)
A two-domain folding intermediate of RuBisCO in complex with the GroEL chaperonin.
International Journal of Biological Macromolecules
, 118
(Part A)
pp. 671-675.
10.1016/j.ijbiomac.2018.06.120.
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Abstract
The chaperonins (GroEL and GroES in Escherichia coli) are ubiquitous molecular chaperones that assist a subset of essential substrate proteins to undergo productive folding to the native state. Using single particle cryo EM and image processing we have examined complexes of E. coli GroEL with the stringently GroE-dependent substrate enzyme RuBisCO from Rhodospirillum rubrum. Here we present snapshots of non-native RuBisCO - GroEL complexes. We observe two distinct substrate densities in the binary complex reminiscent of the two-domain structure of the RuBisCO subunit, so that this may represent a captured form of an early folding intermediate. The occupancy of the complex is consistent with the negative cooperativity of GroEL with respect to substrate binding, in accordance with earlier mass spectroscopy studies.
| Type: | Article |
|---|---|
| Title: | A two-domain folding intermediate of RuBisCO in complex with the GroEL chaperonin |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ijbiomac.2018.06.120 |
| Publisher version: | https://doi.org/10.1016/j.ijbiomac.2018.06.120 |
| Language: | English |
| Additional information: | Copyright © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Protein folding, GroEL, Chaperonin, Non-native protein, RuBisCO, Single particle cryo-EM |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10073455 |
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