Yakimovich, A; Mercer, J; (2019) High-Content Analyses of Vaccinia Plaque Formation. Methods in Molecular Biology , 2023 pp. 237-253. 10.1007/978-1-4939-9593-6_15.

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Abstract

Vaccinia virus plaque assays are employed for quantification of virus titer through serial dilution of virus on a monolayer of cells. Once the virus titer is diluted enough to allow for only few cells of the monolayer to be infected, clonal spread of infection can be detected by observing the lesion in the cell monolayer or using virus specific staining methods. Beyond simple titration, plaque formation bares priceless underlying information about subtle virus-host interactions and their impact on virus spread during multiple rounds of infection. These include virus infectivity, the mode of virus spread, virus replication rate and spatiotemporal spread efficacy. How this underlying information can be harnessed using a high-content imaging setup is discussed here.

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