Bowers, K;
(2019)
RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells.
Methods in Molecular Biology
, 1998
pp. 305-318.
10.1007/978-1-4939-9492-2_22.
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Abstract
Specific depletion of proteins from cultured cells using RNA interference (RNAi) has been a useful technique in assessing protein function for many years. RNAi allows the degradation of specific, targeted mRNA, allowing the effects of protein depletion on cellular processes to be examined. Here, I present a protocol for the depletion of proteins from cultured HeLa cells, and list specific reagents and considerations for targeting the endosomal sorting complexes required for transport (ESCRT).
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