Little, D;
Luft, C;
Mosaku, O;
Ketteler, R;
Devine, MJ;
Gissen, P;
(2019)
High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons.
Methods in Molecular Biology
, 1994
pp. 175-184.
10.1007/978-1-4939-9477-9_16.
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Abstract
Mitochondrial dysfunction is linked to many neurological diseases; therefore, the ability to measure mitochondrial function is of great use for researching disease and testing potential therapeutics. Here we describe a high-content assay to simultaneously measure mitochondrial membrane potential, morphology and cell viability in iPSC-derived neurons. Neurons are seeded into plates suitable for fluorescent microscopy, stained with the mitochondrial membrane potential-dependent dye TMRM, cytoplasmic dye Calcein AM, and nuclear stain Hoechst 33342. Images are acquired in live cells and analyzed using automated image analysis software.
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