Windner, SE;
Manhart, A;
Brown, A;
Mogilner, A;
Baylies, MK;
(2019)
Nuclear Scaling Is Coordinated among Individual Nuclei in Multinucleated Muscle Fibers.
Developmental Cell
, 49
(1)
48-62.e3.
10.1016/j.devcel.2019.02.020.
Preview |
Text
DEVELOPMENTAL-CELL-D-18-00488_R1-1_accepted.pdf - Accepted Version Download (3MB) | Preview |
Abstract
Optimal cell performance depends on cell size and the appropriate relative size, i.e., scaling, of the nucleus. How nuclear scaling is regulated and contributes to cell function is poorly understood, especially in skeletal muscle fibers, which are among the largest cells, containing hundreds of nuclei. Here, we present a Drosophila in vivo system to analyze nuclear scaling in whole multinucleated muscle fibers, genetically manipulate individual components, and assess muscle function. Despite precise global coordination, we find that individual nuclei within a myofiber establish different local scaling relationships by adjusting their size and synthetic activity in correlation with positional or spatial cues. While myonuclei exhibit compensatory potential, even minor changes in global nuclear size scaling correlate with reduced muscle function. Our study provides the first comprehensive approach to unraveling the intrinsic regulation of size in multinucleated muscle fibers. These insights to muscle cell biology will accelerate the development of interventions for muscle diseases.
| Type: | Article |
|---|---|
| Title: | Nuclear Scaling Is Coordinated among Individual Nuclei in Multinucleated Muscle Fibers |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.devcel.2019.02.020 |
| Publisher version: | https://doi.org/10.1016/j.devcel.2019.02.020 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Drosophila, Fibrillarin, cell size, nuclear positioning, nuclear scaling, nuclear synthetic activity, nucleolus, polyploidy, skeletal muscle, syncytial organization |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Mathematics |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10085909 |
Archive Staff Only
 |
View Item |
