Rebane, AA;
Ziltener, P;
LaMonica, LC;
Bauer, AH;
Zheng, H;
López-Montero, I;
Pincet, F;
... Ernst, AM; + view all
(2019)
Liquid-liquid phase separation of the Golgi matrix protein GM130.
FEBS Letters
10.1002/1873-3468.13715.
(In press).
Preview |
Text
Rothman_1873-3468.13715.pdf - Published Version Download (1MB) | Preview |
Abstract
Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50-400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as vesicle tethers, Golgins may also possess the capacity to phase separate and, thereby, contribute to the internal organization of the Golgi. GM130 is the most abundant Golgin at the cis Golgi. Remarkably, overexpressed GM130 forms liquid droplets in cells analogous to those described for numerous intrinsically disordered proteins with low complexity sequences, even though GM130 is neither low in complexity nor intrinsically disordered. Virtually pure recombinant GM130 also phase-separates into dynamic, liquid-like droplets in close to physiological buffers and at concentrations similar to its estimated local concentration at the cis Golgi.
| Type: | Article |
|---|---|
| Title: | Liquid-liquid phase separation of the Golgi matrix protein GM130 |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/1873-3468.13715 |
| Publisher version: | https://doi.org/10.1002/1873-3468.13715 |
| Language: | English |
| Additional information: | © 2019 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Golgi matrix, Golgin, coiled-coil, liquid, liquid phase separation |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10088486 |
Archive Staff Only
 |
View Item |
