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Analysis of Escherichia coli K1 virulence genes by transposon-directed sequencing

McCarthy, AJ; Taylor, P; (2021) Analysis of Escherichia coli K1 virulence genes by transposon-directed sequencing. Essential Genes and Genomes , 2377 pp. 199-213. 10.1007/978-1-0716-1720-5_11. Green open access

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Abstract

Transposon-directed insertion site sequencing (TraDIS) combines random transposon mutagenesis and massively parallel sequencing to shed light on bacterial gene function on a genome-wide scale and in a high-throughput manner. The technique has proven to be successful in the determination of the fitness contribution of every gene under specific conditions both in vitro and in vivo. In this contribution, we describe the procedure used for the identification of Escherichia coli K1 genes essential for in vitro growth, survival in pooled human serum and gastrointestinal colonisation in a rodent model of neonatal invasive infection. TraDIS has broad application for systems-level analysis of a wide range of pathogenic, commensular and saprophytic bacteria.

Type: Article
Title: Analysis of Escherichia coli K1 virulence genes by transposon-directed sequencing
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-0716-1720-5_11
Publisher version: https://doi.org/10.1007/978-1-0716-1720-5_11
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Transposon-directed insertion site sequencing, Essential genes, Escherichia coli, Systems microbiology, Bacterial pathogenesis, Bacterial fitness
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10089800
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