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The cell biologist's guide to super-resolution microscopy

Jacquemet, G; Carisey, AF; Hamidi, H; Henriques, R; Leterrier, C; (2020) The cell biologist's guide to super-resolution microscopy. Journal of Cell Science , 133 (11) , Article jcs240713. 10.1242/jcs.240713. Green open access

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Abstract

Fluorescence microscopy has become a ubiquitous method to observe the location of specific molecular components within cells. However, the resolution of light microscopy is limited by the laws of diffraction to a few hundred nanometers, blurring most cellular details. Over the last two decades, several techniques – grouped under the ‘super-resolution microscopy’ moniker – have been designed to bypass this limitation, revealing the cellular organization down to the nanoscale. The number and variety of these techniques have steadily increased, to the point that it has become difficult for cell biologists and seasoned microscopists alike to identify the specific technique best suited to their needs. Available techniques include image processing strategies that generate super-resolved images, optical imaging schemes that overcome the diffraction limit and sample manipulations that expand the size of the biological sample. In this Cell Science at a Glance article and the accompanying poster, we provide key pointers to help users navigate through the various super-resolution methods by briefly summarizing the principles behind each technique, highlighting both critical strengths and weaknesses, as well as providing example images.

Type: Article
Title: The cell biologist's guide to super-resolution microscopy
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/jcs.240713
Publisher version: https://doi.org/10.1242/jcs.240713
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Super-resolution microscopy, Live-cell imaging, Labeling, Sample preparation
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery-pp.ucl.ac.uk/id/eprint/10102450
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