Gutowski, Nicholas Jan; (1995) Cellular and molecular studies related to a cell line of the oligodendrocyte-type-2 astrocyte lineage derived from a human glioblastoma multiforme. Doctoral thesis (M.D), UCL (University College London).

Preview

Text Cellular_and_molecular_studies.pdf Download (12MB) | Preview

Abstract

By using techniques from studies of the rodent oligodendrocyte-type-2 astrocyte (0-2A) lineage, the Human 0-2A lineage Glioblastoma number 1 (Hu-O-2A/Gbl) cell line was grown from a human glioblastoma multiforme. This thesis describes findings related to this cell line. Receptor tyrosine kinases and other tyrosine kinase complementary DNA fragments were cloned from the cell line to provide information about growth factors to which the cells might respond. Consequently, it was found that the cells showed mitogenic and antigenic changes in several culture conditions, similar to that seen for rodent 0-2A progenitor cells. The cloning data also suggested that Vascular Endothelial Growth Factor (VEGF) might affect these cells. It was found that VEGF enhanced oligodendrocyte maturation in the Hu-0-2A/Gbl cell line and also in rodent oligodendrocytes in vitro. In addition, the HEK2 receptor tyrosine kinase found during initial cloning from the Hu-0-2A/Gbl cell line appears to be a helpful, but not entirely specific, marker for 0-2A lineage cells. A new protein kinase LIMK was cloned from the complementary DNA library of the Hu-0-2A/Gbl cell line. LIMK is predominantly expressed in the nervous system and high expression was found in malignant glioma cell populations, their original tumours and also during human embryonic development.

Download activity - last month

Export as JSONXMLCSV

Download activity - last 12 months

Export as CSVXMLJSON

Downloads by country - last 12 months

Export as CSVJSONXML

Archive Staff Only

View Item