Cao, L;
Yonis, A;
Vaghela, M;
Barriga, EH;
Chugh, P;
Smith, MB;
Maufront, J;
... Charras, G; + view all
(2020)
SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization.
Nature Cell Biology
, 22
pp. 803-814.
10.1038/s41556-020-0531-y.
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Text (Accepted Manuscript)
Charras_SPIN90 associates with mDia1 and the Arp2:3 complex to regulate cortical actin organization_AAM.pdf - Accepted Version Download (3MB) | Preview |
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Text (Supplementary Material)
Charras_SPIN90 associates with mDia1 and the Arp2:3 complex to regulate cortical actin organization_SuppM.pdf - Accepted Version Download (3MB) | Preview |
Abstract
Cell shape is controlled by the submembranous cortex, an actomyosin network mainly generated by two actin nucleators: the Arp2/3 complex and the formin mDia1. Changes in relative nucleator activity may alter cortical organization, mechanics and cell shape. Here we investigate how nucleation-promoting factors mediate interactions between nucleators. In vitro, the nucleation-promoting factor SPIN90 promotes formation of unbranched filaments by Arp2/3, a process thought to provide the initial filament for generation of dendritic networks. Paradoxically, in cells, SPIN90 appears to favour a formin-dominated cortex. Our in vitro experiments reveal that this feature stems mainly from two mechanisms: efficient recruitment of mDia1 to SPIN90-Arp2/3 nucleated filaments and formation of a ternary SPIN90-Arp2/3-mDia1 complex that greatly enhances filament nucleation. Both mechanisms yield rapidly elongating filaments with mDia1 at their barbed ends and SPIN90-Arp2/3 at their pointed ends. Thus, in networks, SPIN90 lowers branching densities and increases the proportion of long filaments elongated by mDia1.
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