Hammond, K;
Lewis, H;
Halliwell, S;
Desriac, F;
Nardone, B;
Ravi, J;
Hoogenboom, BW;
... Ryadnov, MG; + view all
(2020)
Flowering poration – a synergistic multi-mode antibacterial mechanism by a bacteriocin fold.
iScience
, Article 101423. 10.1016/j.isci.2020.101423.
(In press).
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Abstract
Bacteriocins are a distinct family of antimicrobial proteins postulated to porate bacterial membranes. However, direct experimental evidence of pore formation by these proteins is lacking. Here we report a multi-mode poration mechanism induced by four-helix bacteriocins, epidermicin NI01 and aureocin A53. Using a combination of crystallography, spectroscopy, bioassays and nanoscale imaging, we established that individual two-helix segments of epidermicin retain antibacterial activity but each of these segments adopts a particular poration mode. In the intact protein these segments act synergistically to balance out antibacterial and hemolytic activities. The study sets a precedent of multi-mode membrane disruption advancing the current understanding of structure-activity relationships in pore-forming proteins.
| Type: | Article |
|---|---|
| Title: | Flowering poration – a synergistic multi-mode antibacterial mechanism by a bacteriocin fold |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.isci.2020.101423 |
| Publisher version: | https://doi.org/10.1016/j.isci.2020.101423 |
| Language: | English |
| Additional information: | © 2020 The Author(s). Published by Elsevier under a Creative Commons license (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10106730 |
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