Aprile, FA;
Sormanni, P;
Podpolny, M;
Chhangur, S;
Needham, L-M;
Ruggeri, FS;
Perni, M;
... Vendruscolo, M; + view all
(2020)
Rational design of a conformation-specific antibody for the quantification of A beta oligomers.
Proceedings of the National Academy of Sciences
, 117
(24)
pp. 13509-13518.
10.1073/pnas.1919464117.
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Abstract
The accurate quantification of the amounts of small oligomeric assemblies formed by the amyloid β (Aβ) peptide represents a major challenge in the Alzheimer’s field. There is therefore great interest in the development of methods to specifically detect these oligomers by distinguishing them from larger aggregates. The availability of these methods will enable the development of effective diagnostic and therapeutic interventions for this and other diseases related to protein misfolding and aggregation. We describe here a single-domain antibody able to selectively quantify oligomers of the Aβ peptide in isolation and in complex protein mixtures from animal models of disease.
| Type: | Article |
|---|---|
| Title: | Rational design of a conformation-specific antibody for the quantification of A beta oligomers |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1073/pnas.1919464117 |
| Publisher version: | https://doi.org/10.1073/pnas.1919464117 |
| Language: | English |
| Additional information: | This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND). |
| Keywords: | Alzheimer’s disease, amyloid, protein aggregation, protein design |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10106995 |
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