Yang, T;
Cheong, A;
Mai, X;
Zou, S;
Woon, ECY;
(2016)
A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity.
Chemical Communications
, 52
(36)
pp. 6181-6184.
10.1039/c6cc01045h.
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Abstract
We describe a novel methylation-sensitive nucleic acid (RNA) probe which switches conformation according to its methylation status. When combined with a differential scanning fluorimetry technique, it enables highly sensitive and selective detection of demethylase activity at a single methylated-base level. The approach is highly versatile and may be adapted to a broad range of RNA demethylases.
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