Haas, TM;
Qiu, D;
Häner, M;
Angebauer, L;
Ripp, A;
Singh, J;
Koch, H-G;
... Jessen, HJ; + view all
(2020)
Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis.
The Journal of Organic Chemistry
, 85
(22)
pp. 14496-14506.
10.1021/acs.joc.0c00841.
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Abstract
The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.
| Type: | Article |
|---|---|
| Title: | Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1021/acs.joc.0c00841 |
| Publisher version: | https://doi.org/10.1021/acs.joc.0c00841 |
| Language: | English |
| Additional information: | © 2020 This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html). |
| Keywords: | Nucleic acids, Peptides and proteins, Monomers,Ketones, Cations |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10113618 |
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