Macdonald, NP;
Menachery, A;
Reboud, J;
Cooper, JM;
(2018)
Creating tissue on chip constructs in microtitre plates for drug discovery.
RSC Advances
, 8
(18)
pp. 9603-9610.
10.1039/c8ra00849c.
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Abstract
We report upon a novel coplanar dielectrophoresis (DEP) based cell patterning system for generating transferrable hepatic cell constructs, resembling a liver-lobule, in culture. The use of paper reinforced gel substrates provided sufficient strength to enable these constructs to be transfered into 96-well plates for long term functional studies, including in the future, drug development studies. Experimental results showed that hepatic cells formed DEP field-induced structures corresponding to an array of lobulemimetic patterns. Hepatic viability was observed over a period of 3 days by the use of a fluorescent cell staining technique, whilst the liver specific functionality of albumin secretion showed a significant enhancement due to the layer patterning of cell lines (HepG2/C3A), compared to 2D patterned cells and un-patterned control. This “build and transfer” concept could, in future, also be adapted for the layer-bylayer construction of organs-on-chip in microtitre formats.
| Type: | Article |
|---|---|
| Title: | Creating tissue on chip constructs in microtitre plates for drug discovery |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/c8ra00849c |
| Publisher version: | https://doi.org/10.1039/c8ra00849c |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. http://creativecommons.org/licenses/by/3.0/ |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10114424 |
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