Wang, Y;
Sun, S;
Luo, J;
Xiong, Y;
Ming, T;
Liu, J;
Ma, Y;
... Cai, X; + view all
(2020)
Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR.
Microsystems and Nanoengineering
, 6
(1)
, Article 32. 10.1038/s41378-020-0146-2.
(In press).
Preview |
Text
s41378-020-0146-2.pdf - Published Version Download (1MB) | Preview |
Abstract
In this work, an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor (EGFR) by employing anti-EGFR aptamers as the bio-recognition element. The device used the concept of paper-folding, or origami, to serve as a valve between sample introduction and detection, so reducing sampling volumes and improving operation convenience. Amino-functionalized graphene (NH2-GO)/thionine (THI)/gold particle (AuNP) nanocomposites were used to modify the working electrode not only to generate the electrochemical signals, but also to provide an environment conducive to aptamer immobilization. Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode, thus resulting in a lower signal. The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL−1 (R2 = 0.989) and a detection limit of 5 pgmL−1 for EGFR. The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples, showing good agreement with the gold-standard enzyme-linked immunosorbent assay.
| Type: | Article |
|---|---|
| Title: | Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41378-020-0146-2 |
| Publisher version: | http://dx.doi.org/10.1038/s41378-020-0146-2 |
| Language: | English |
| Additional information: | © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| Keywords: | Biosensors, Microfluidics, Nanoparticles |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10114497 |
Archive Staff Only
 |
View Item |
