Afshar, A;
Yuca, E;
Wisdom, C;
Alenezi, H;
Ahmed, J;
Tamerler, C;
Edirisinghe, M;
(2021)
Next‐generation Antimicrobial Peptides (AMPs) incorporated nanofibre wound dressings.
Medical Devices & Sensors
, 4
(1)
, Article e10144. 10.1002/mds3.10144.
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Abstract
Antimicrobial peptides (AMPs) containing polymer-based nanodelivery systems offer to overcome many challenges in wound care. While preventing the contact of the external agents on the wound, it also addresses a rising concern on the drug resistance. AMPs as the host defence peptides have been increasingly recognized for therapeutic potential owning to their critical role in innate immunity. Here we investigated a nanofibre mesh approach using AMPs incorporated polyethylene oxide (PEO) for wound healing applications. PEO was prepared to carry GH12-COOH-M2 (type 1 AMP) and AMP2 (type 2 AMP), and their antibacterial activity was assessed against Staphylococcus epidermidis (S. epidermidis). PEO-AMP nanofibre meshes were successfully formed by using pressurized gyration (PG), which allows rapid mass production. Bacterial viability of the nanofibre meshes was investigated using the AlamarBlue assay. Fibre morphology, size distribution and AMP incorporation in the nanofibres were characterized by scanning electron microscopy (SEM), fluorescence microscopy (polarization contrast images) and Fourier transform infrared spectroscopy (FTIR). While both PEO-AMP1 and PEO-AMP2 nanofibres indicate promising bacterial inhibition at 105 µg/ml, PEO-AMP2 fibres showed the highest S. epidermidis reduction. The results demonstrated that increase in the AMP content reduced the bacterial growth. Another important implementation of the PEO-AMP nanofibres is that they can be tuned to rapidly releasing the peptides. Antimicrobial peptide-loaded nanofibres represent a viable biologically active solution to next-generation wound dressings.
| Type: | Article |
|---|---|
| Title: | Next‐generation Antimicrobial Peptides (AMPs) incorporated nanofibre wound dressings |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/mds3.10144 |
| Publisher version: | http://dx.doi.org/10.1002/mds3.10144 |
| Language: | English |
| Additional information: | © 2020 The Authors. Medical Devices & Sensors published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | antimicrobial peptides, fibre, pressurized gyration, staphylococcus epidermidis, wound healing |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10126727 |
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