Pape, J;
Emberton, M;
Cheema, U;
(2021)
3D Cancer Models: The Need for a Complex Stroma, Compartmentalization and Stiffness.
Frontiers in Bioengineering and Biotechnology
, 9
, Article 660502. 10.3389/fbioe.2021.660502.
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Abstract
The use of tissue-engineered 3D models of cancer has grown in popularity with recent advances in the field of cancer research. 3D models are inherently more biomimetic compared to 2D cell monolayers cultured on tissue-culture plastic. Nevertheless 3D models still lack the cellular and matrix complexity of native tissues. This review explores different 3D models currently used, outlining their benefits and limitations. Specifically, this review focuses on stiffness and collagen density, compartmentalization, tumor-stroma cell population and extracellular matrix composition. Furthermore, this review explores the methods utilized in different models to directly measure cancer invasion and growth. Of the models evaluated, with PDX and in vivo as a relative "gold standard", tumoroids were deemed as comparable 3D cancer models with a high degree of biomimicry, in terms of stiffness, collagen density and the ability to compartmentalize the tumor and stroma. Future 3D models for different cancer types are proposed in order to improve the biomimicry of cancer models used for studying disease progression.
| Type: | Article |
|---|---|
| Title: | 3D Cancer Models: The Need for a Complex Stroma, Compartmentalization and Stiffness |
| Location: | Switzerland |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fbioe.2021.660502 |
| Publisher version: | https://doi.org/10.3389/fbioe.2021.660502 |
| Language: | English |
| Additional information: | © 2021 Pape, Emberton and Cheema. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | 3D models, collagen density, compartmentalization, extracellular matrix, stiffness, stromal cells, tissue-engineering, tumor stroma |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/10126898 |
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